Urine PH Test

pH tests for the presence of acidic or alkaline adulterants in urine. Normal pH levels should be in the range of 4.0 to 9.0. Values outside of this range may indicate the urine sample has been altered or there may be a problem that needs medical attention.

Why the pH test is performed

Urine pH is a measure of how acidic or alkaline a urine sample. A urine pH of 4 is strongly acidic, 7 is neutral (neither acidic nor alkaline), and 9 is strongly alkaline. Sometimes the pH of urine is affected by certain treatments. For example, your doctor may instruct you how to keep your urine either acidic or alkaline to prevent some types of kidney stones from forming.

The pH in blood is maintained within the narrow range of 7.35 to 7.45. Your body maintains this narrow range by using buffers -- chemicals that can switch back and forth between 2 forms, a weak acid or weak base. The buffers are only temporary "stop gaps" to avoid dramatic changes in blood pH.

Your provider may want to test your urine pH to see if you are at risk for kidney stones. Acidic urine is associated with xanthine, cystine, uric acid, and calcium oxalate stones. Alkaline urine is associated with calcium carbonate, calcium phosphate, and magnesium phosphate stones.

Your doctor may also order this test if you need to take certain medications. Some medications are more effective in acidic or alkaline environments. For example, streptomycin, neomycin, and kanamycin are more effective in treating urinary tract infections when the urine is alkaline.

In some cases, checking your urine pH is helpful for identifying body acid-base imbalances. In other cases, a blood pH test is needed.

Some medications are more effective in acidic or alkaline environments. For example, streptomycin, neomycin, and kanamycin are more effective in treating urinary tract infections when the urine is alkaline.

Normal Values: The normal urine pH values range from 4.6 to 8.0.

What abnormal results mean:

A high urine pH (alkaline urine) may indicate: Gastric suction, Renal or Kidney failure, Renal or Kidney tubular acidosis, Urinary tract infection , Vomiting

A low urine pH (acidic urine) may indicate: Chronic obstructive pulmonary disease (such as emphysema), Diabetic ketoacidosis, Diarrhea, Starvation

The test also may be performed to investigate: Alkalosis, Interstitial nephritis , Renal tubular acidosis - distal, Sepsis

How the pH test is performed

Collect a "clean-catch" (midstream) urine sample. To obtain a clean-catch sample, men or boys should wipe clean the head of the penis. Women or girls need to wash the area between the lips (labia) of the vagina with soapy water and rinse well.

As you start to urinate, allow a small amount to fall into the toilet bowl (this clears the urethra of contaminants). Then, in a clean container or sterile container, catch about 1 to 2 ounces of urine and remove the container from the urine stream. Remove the container from the urine stream without stopping the flow. You may finish urinating into the toilet bowl. Then, either give the urine sample person performing the test or set it aside if performing the test yourself.

For infants, the genital area is cleaned and dried, and then a collection device is attached to collect the urine. Open a urine collection bag (a plastic bag with an adhesive paper on one end), and place it on your infant. For boys, the entire penis can be placed in the bag and the adhesive attached to the skin. For girls bag is placed over the labia. Place a diaper over the infant (bag and all). After your baby has urinated, the urine (at least 20 cc) is placed in a sterile container.

Check your baby frequently and remove the bag after the infant has urinated into it. For active infants, this procedure may take a couple of attempts -- lively infants can displace the bag. The urine is drained into a container for transport back to the health care provider.

How to prepare for the test

If the test will be conducted by your health care provider, he or she may advise you to stop taking certain drugs that can affect the results of the test. Do not stop taking any prescribed medication unless advised to do so by a health profession (like your doctor).

Eat a normal, balanced diet for several days before the test. Eat a normal, balanced diet for several days before the test.

  • A diet high in citrus fruits, vegetables, or dairy products can increase your urine pH.

  • A diet high in meat products or cranberries can decrease your urine pH.

If the specimen is contaminated by vaginal discharge or bleeding, then collect a clean-catch sample. If the collection is being taken from an infant, a couple of extra collection bags may be necessary. For more information on how to obtain a clean-catch sample, see How The Test Is Performed section.

How the test will feelThe test involves only normal urination, and there is no discomfort.

What the risks are! There are no risks.

Special considerations:

Urine pH can be affected by various factors after collection such as leaving the urine standing in an uncovered container. Bacteria usually increase the pH as they break down urea in the urine to ammonia.

A diet high in citrus fruits, vegetables, or dairy products can increase your urine pH. Some drugs also can increase urine pH, including acetazolamide, potassium citrate, and sodium bicarbonate.

A diet high in meat products or cranberries can decrease your urine pH. Drugs that can decrease urine pH include ammonium chloride, chlorothiazide diuretics, and methenamine mandelate.

Urine pH can be affected by various factors. For more information on a urine test, see Urinalysis Test or visit UATest.com. Other related resources: Protein Urine, complete list of urine dipstick tests, urine drug test.

7 Parameter Urine Test - pH Test


#DUC-111 - One Step Specimen Validity Test (SVT) (25 tests per bottle).

Urine Adulteration Test Strips provide rapid, semi-quantitative detection of 7 parameters (Creatinine, Nitrite, Glutaraldehyde, pH, Specific Gravity, Oxidants /

pyridinium chlorochromate(PCC) in human urine. This is sometimes referred to as a chemstrip test.

  • 7 testing parameters

  • Easy to interpret color chart

  • 2 year shelf life

  • 1 minute read time

RETAIL $39.95 Our Price Only $ 17.95 each (bottle of 25).


A rapid, one step screening test for the simultaneous detection of Creatinine, Nitrite, Glutaraldehyde, pH, Specific Gravity, and Oxidants/Pyridinium Chlorochromate (PCC) in human urine.

For forensic use only.


The One Step S.V.T. is a semi-quantitative, color comparison screen for the detection of pH, creatinine, nitrite, glutaraldehyde, specific gravity, and oxidants / pyridinium . chlorochromate (PCC) in human urine.

This test provides a preliminary screen only. A more specific alternate chemical method must be used in order to obtain a confirmed analytical result. Abnormal results should be sent to a laboratory for confirmation.


Each of the plastic strips contains six (6) chemically treated reagent pads. One (1) minute following the activation of the reagent pads by the urine sample, the colors that appear on the pads can be compared with the printed color chart on the canister. The color comparison provides a semi-quantitative screen for creatinine, nitrite, glutaraldehyde, pH, specific gravity, and oxidants / pyridinium chlorochromate (PCC) in human urine which can help assess the integrity of the urine sample.

What is adulteration?

Adulteration is the tampering of a urine specimen with the intention of altering the test results. The use of adulterants can cause false negative results in drug tests by either interfering with the screening test and/ or destroying the drugs present in the urine. Dilution may also be employed in an attempt to produce false negative drug test results.

One of the best ways to test for adulteration or dilution is to determine certain urinary characteristics such as creatinine, pH, and specific gravity and to detect the presence of glutaraldehyde, nitrite and oxidants /pyridinium chlorochromate (PCC) in urine.

  • Creatinine is a waste product of creatine; an amino-acid contained in muscle tissue and found in urine.1 A person may attempt to foil a test by drinking excessive amounts of water or diuretics such as herbal teas to “flush” the system. Creatinine and specific gravity are two ways to check for dilution and flushing, which are the most common mechanisms used in an attempt to circumvent drug testing. Low creatinine and specific gravity levels may indicate dilute urine. The absence of creatinine (<5mg/dl) is indicative of a specimen not consistent with human urine.

  • Specific gravity tests for sample dilution. The normal range is from 1.003 to 1.030. Values outside this range may be the result of specimen dilution or adulteration.

  • Nitrite tests for commonly used commercial adulterants such as Klear or Whizzies. They work by oxidizing the major cannabinoid metabolite THC-COOH.2 Normal urine should contain no trace of nitrite. Positive results generally indicate the presence of an adulterant.

  • Glutaraldehyde tests for the presence of an aldehyde. Adulterants such as UrinAid and Clear Choice contain glutaraldehyde which may cause false negative screening results by disrupting the enzyme used in some immunoassay tests. Glutaraldehyde is not normally found in urine; therefore, detection of glutaraldehyde in a urine specimen is generally an indicator of adulteration.

  • pH tests for the presence of acidic or alkaline adulterants in urine. Normal pH levels should be in the range of 4.0 to 9.0. Values outside of this range may indicate the sample has been altered.

  • Oxidants/PCC (Pyridinium Chlorochromate) tests for the presence of oxidizing agents such as bleach and hydrogen peroxide. Pyridinium chlorochromate (sold under the brand name UrineLuck) is a commonly used adulterant. Normal human urine should not contain oxidants or PCC.


The test is based on the color derived from the chemical reaction between the chemical reagent on each test pad and the urine sample.


Adulteration Pad

Reactive indicator

Buffers and non-reactive ingredients













Specific Gravity



Oxidants / PCC





  • For forensic use only. Do not use after the expiration date.

  • The adulteration strips should remain in the sealed canister until use.

  • All specimens should be considered potentially hazardous and handled in the same manner as an infectious agent.

  • The used test strip should be discarded according to federal, state and local regulations.


Store as packaged in the sealed canister at 15-30C. The test strips must remain sealed in the canister until use. DO NOT FREEZE. Do not use beyond the expiration date. Avoid direct exposure to sunlight.


Urine Assay

The urine specimen must be collected in a clean and dry container. Test urine as soon as possible after collection.

Specimen Storage

For best results, test specimens immediately following collection. Storage of urine specimens should not exceed 2 hours at room temperature or 4 hours refrigerated (2-8C) prior to testing.


Materials Provided

  • Each canister contains 25 S.V.T. Strips

  • Package insert

Materials Required But Not Provided

  • Timer


  1. Allow the adulteration strip to equilibrate to room temperature (15-30 C) prior to testing.

  2. Remove the strip(s) from the canister and recap tightly.

  3. Dip test strip into the urine specimen and remove immediately.

  4. Blot the test gently on its side to remove excess urine. NOTE: It is important to blot the test strip for consistent results.

  5. Read results in one (1) minute by comparing each pad with the color chart printed on the canister. Do not interpret test results after 4 minutes.

  6. If the test indicates adulteration, refer to your Drug Free Policy for guidelines on handling adulterated specimens.


(Please refer to the illustration above)

Semi Quantitative results are obtained by visually comparing the reacted color blocks on the strip to the printed color blocks on the canister. No instrumentation is required.

Quality Control

Control standards are not supplied with this kit. However, it is recommended that positive and negative specimens or controls be tested as good laboratory practice to confirm the test procedure and to verify proper test performance.


  1. The adulteration tests included with this product are meant to aid in the determination of abnormal specimens. While comprehensive, these tests are not meant to be an “all-inclusive” representation of possible adulterants.

  2. Creatinine: Normal creatinine levels are between 20 and 350 mg/dL. Under rare conditions, certain kidney diseases may show dilute urine.

  3. Nitrite: Nitrite is not a normal component of human urine. However, nitrite found in urine may indicate urinary tract infections or bacterial infections. Nitrite levels of > 20 mg/dL may produce false positive glutaraldehyde results.

  4. Glutaraldehyde: Is not normally found in urine. However certain metabolic abnormalities such as ketoacidosis (fasting, uncontrolled diabetes or high-protein diets) may interfere with the test results.

  5. Specific Gravity: Elevated levels of protein in urine may cause abnormally high specific gravity values.

  6. Oxidants/PCC: Normal human urine should not contain oxidants or PCC. The presence of high levels of antioxidants in the specimen, such as ascorbic acid, may result in false negative results for the oxidants/PCC pad.

Home Drug Test


  1. Tietz NW. Textbook of Clinical Chemistry. W.B. Saunders Company. 1986, 1734.

  2. Tsai, S.C. et.al., J. Anal. Toxicol. 1998; 22 (6): 474

  3. Cody, J.T., “Specimen Adulteration in drug urinalysis. Forsenic Sci. Rev., 1990, 2:63.

  4. Mikkelsen, S.L. et.al., Clin. Chem. 1988; 34: 648

  5. Hardman J, Limbird LE (Eds). Goodman & Gilman’s The Pharmacological Basis of Therapeutics, 10th Ed., McGraw-Hill Publishing. 2001, 1010.

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